DB-ALM News

03-03-08

We have the pleasure to inform you that a first set of information sheets have been finalised by the DB-ALM and its collaborators and are now made available online for the topic area of:

 Hepatotoxicity/Metabolism-Mediated toxicity.

The following descriptions of in-vitro techniques using the below listed experimental systems have been either updated or newly added to the DB-ALM content. They include information on their objectives & applications, rationale, basic procedure and status of development and/or validation and all are based on extensive literature reviews. For each technique related information is also provided.

List of new and/or updated information sheets available online:                                              

·        Alginate entrapped primary hepatocytes (LiverBeadsTM) – Summary                   

·        Collagen-gel sandwich configuration culture of primary hepatocytes - Summary               

·        Culture of freshly isolated nonparenchymal liver cells and nonparenchymal cell lines - Summary                                                          

·        Hepatocyte spheroid culture using primary hepatocytes or hepatic cell lines - Summary

·        Incubation techniques for the use of liver slices - Summary                                

·        Isolated hepatocyte couplets - Summary

·        Isolated perfused liver - Summary                                     

·        Monolayer culture of primary hepatocytes - Summary                             

·        Preparation of precision-cut liver slices - Summary                                                     

·        Preservation of liver slices - Summary                                                  

·        Suspension culture of freshly isolated or cryopreserved hepatocytes - Summary                               

·        The use of liver slices for hepatotoxicity studies- Summary                                          

We invite you to consult the whole sector.

For more information: dbalm-contact@jrc.it

The DB-ALM

European Commission - Joint Research Centre (EC-JRC)
Institute for Health and Consumer Protection (IHCP)
European Centre for the Validation of Alternative Methods (ECVAM)